The association of thymidine kinase activity and thymidine transport in Escherichia coli
Identifieur interne : 004898 ( Main/Exploration ); précédent : 004897; suivant : 004899The association of thymidine kinase activity and thymidine transport in Escherichia coli
Auteurs : Dipak K. Dube [États-Unis] ; Marshall S. Z. Horwitz [États-Unis] ; Lawrence A. Loeb [États-Unis]Source :
- Gene [ 0378-1119 ] ; 1991.
English descriptors
- Teeft :
Abstract
Abstract: We have constructed a series of mutants within the putative nucleoside-binding site of the herpes simplex type-1 virus (HSV-1) thymidine kinase (TK)-encoding gene (tk), contained within an expression vector. While most mutations within this sequence produce an inactive protein, we find no absolute requirement for the wild-type He166 and Ala167. The uptake of thymidine (dT) into Escherichia coli tdk−, lacking functional endogenous TK activity, is proportional to the amount of TK activity expressed from the heterologous HSV-1 tk gene. In contrast, there is no enhancement in deoxycytidine uptake into E. coli producing (HSV-1) TK. These results imply a specific role for TK in the active transport of dT into E. coli.
Url:
DOI: 10.1016/0378-1119(91)90029-B
Affiliations:
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Dube</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Pathology, Joseph Gottstein Memorial Cancer Research Laboratory, University of Washington, Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Horwitz, Marshall S Z" sort="Horwitz, Marshall S Z" uniqKey="Horwitz M" first="Marshall S. Z." last="Horwitz">Marshall S. Z. Horwitz</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Pathology, Joseph Gottstein Memorial Cancer Research Laboratory, University of Washington, Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Loeb, Lawrence A" sort="Loeb, Lawrence A" uniqKey="Loeb L" first="Lawrence A." last="Loeb">Lawrence A. Loeb</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Pathology, Joseph Gottstein Memorial Cancer Research Laboratory, University of Washington, Seattle</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">99</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="25">25</biblScope><biblScope unit="page" to="29">29</biblScope></imprint><idno type="ISSN">0378-1119</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Active transport</term><term>Cold spring</term><term>Coli</term><term>Expression vector</term><term>Final concentration</term><term>Herpes</term><term>Kinase</term><term>Kinase activity</term><term>Mutant</term><term>Mutation</term><term>Nucleoside</term><term>Plasmid</term><term>Putative</term><term>Putative nucleosidebinding site</term><term>Putative site</term><term>Simplex</term><term>Single site</term><term>Thymidine</term><term>Uptake</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have constructed a series of mutants within the putative nucleoside-binding site of the herpes simplex type-1 virus (HSV-1) thymidine kinase (TK)-encoding gene (tk), contained within an expression vector. While most mutations within this sequence produce an inactive protein, we find no absolute requirement for the wild-type He166 and Ala167. The uptake of thymidine (dT) into Escherichia coli tdk−, lacking functional endogenous TK activity, is proportional to the amount of TK activity expressed from the heterologous HSV-1 tk gene. In contrast, there is no enhancement in deoxycytidine uptake into E. coli producing (HSV-1) TK. These results imply a specific role for TK in the active transport of dT into E. coli.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Dube, Dipak K" sort="Dube, Dipak K" uniqKey="Dube D" first="Dipak K." last="Dube">Dipak K. Dube</name></region><name sortKey="Horwitz, Marshall S Z" sort="Horwitz, Marshall S Z" uniqKey="Horwitz M" first="Marshall S. Z." last="Horwitz">Marshall S. Z. Horwitz</name><name sortKey="Loeb, Lawrence A" sort="Loeb, Lawrence A" uniqKey="Loeb L" first="Lawrence A." last="Loeb">Lawrence A. Loeb</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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